Clustering app cytoscape4/30/2023 ![]() Therefore, the current research on the pathogenesis of T-ALL mainly includes the following components: PI3K-AKT-mTOR, IL7R, BRD4/MYC, NOTCH1, BCL2, Cyclin D3 (CDK4/CDK6), SINE, and rationale combinations of above approaches. The pathogenesis of T-ALL is a complex network, which involves the regulation and mutual regulation of multiple signaling pathways. Chemotherapy, the only treatment approach currently used by the vast majority of patients, is used to reduce or even completely relieve symptoms by administering chemical small molecule drugs to the patient to kill leukemia cells. Allogenic bone marrow transplantation is currently the only way to cure the disease, but treatment opportunities are extremely low and expensive. The method of biotechnology treatment is to change the genetic information in the patient’s body to achieve therapeutic purposes by means of “gene editing”, which is a promising approach, but the safety and stability of the method remains to be observed. Current treatments for T-ALL patients include: (1) biotechnological treatment, (2) bone marrow transplantation, and (3) chemotherapy drugs. The clinical symptoms of patients with T-ALL are variable, including the large mediastinal mass, high white blood cells count, central nervous system (CNS) invasion and organomegaly. It invades the lymphoid-cell-generating stem cells, in particular a type of white blood cell named T lymphocytes. T-cell acute lymphoblastic leukemia (T-ALL) is a type of acute leukemia that progresses rapidly. Jurkat cells are a leukemia cell line isolated from the peripheral blood of a boy with acute T lymphocytic leukemia, which greatly facilitates the study of the T-cell acute lymphoblastic leukemia (T-ALL). Among the many subtypes of leukemia, acute leukemia accounts for more than 70% of children. Clinically, leukemia is generally classified into acute and chronic leukemia. From a pathological point of view, the type of leukemia is mainly distinguished by the type of immature white blood cells in the blood. Therefore, the treatment of leukemia is one of the key issues that needs to be solved urgently in the medical field.Īs a malignant disease of the blood system, leukemia has a huge classification system. Leukemia is the most common type of cancer for teenagers before the age of 14, and approximately 92% of leukemia patients are diagnosed before the age of 20. ![]() ![]() In 2019, there will be 61,780 new leukemia patients in the United States. ![]() At present, the incidence of leukemia in the world is gradually increasing, which seriously affects the labor force and imposes a heavy burden on medical care in various countries. Due to the lack of normal blood cells, leukemia patients often have symptoms including fever, feeling tired, bleeding and bruising, and are prone to infections. Leukemia is a malignant cancer of blood cells that usually starts in the bone marrow and results in excessive numbers of aberrant blood cells. In conclusion, we explored the effect of nsPEF on Jurkat cell signaling pathway from the perspective of molecular informatics, which will be helpful in understanding the complex effects of nsPEF on acute T-cell leukemia Jurkat cells. Molecular dynamic analysis, including root mean square deviation (RMSD), root mean square fluctuation (RMSF), and the R g, demonstrated that the 3D structure of hub proteins was built, and the structural characteristics of hub proteins under different nsPEFs were acquired. A total of 1769 DEGs and eight hub genes were obtained. Finally, three kinds of nsPEF voltages (0.01, 0.05, and 0.5 mV/mm) were used to simulate the molecular dynamics of hub proteins for 100 ns. Then, a 100 ns molecular dynamics simulation for each hub protein was performed with GROMACS 2018.2. Subsequently, 3D protein models of hub genes were modeled by Modeller 9.21 and Rosetta 3.9. Differentially expressed genes (DEGs) were acquired from the GEO2R, followed by analysis with a series of bioinformatics tools. Here, we explored the cellular processes of Jurkat cells exposed to nanosecond pulsed electric field. Recently, nanosecond pulsed electric field (nsPEF) has been considered as a new tool for tumor therapy, but its molecular mechanism of function remains to be fully elucidated.
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